CD4 and CD8 T cell subsets post vaccination.

<p>One week after the second and final immunization splenocytes were analyzed for intracellular cytokine production by CD4 or CD8 T cells following stimulation with Ag85B (A) or TB10.4 (B). Ag85B was used to evaluate CD4 T cell responses since the highest CD4 T cell vaccine responses were directed against Ag85B shown in A, whereas the highest CD8 T cell responses were directed against TB10.4 shown in B. Mice receiving MVA28 only were given one immunization as this was the optimal regimen for this vector, not shown. The single MVA28 immunization was given simultaneously with the second vaccination. Bars represent percentages of CD4 or CD8 T cells from individual spleens from N = 4 mice per group producing any combination of IFN-γ, TNF-α or IL-2 as indicated below the graphs. Background levels obtained in media-stimulated samples have been deducted. Pie charts represent the relative distribution within the antigen specific CD4 or CD8 T cells pools of subsets producing different cytokine combinations as shown in the histograms.