Silenced transgenes can be reactivated by treatment with the DNA methylase inhibitor, 5′-aza-2′-deoxycitidine (5-AzadC).

<p>(A) 5-AzadC treatment reactivates the silenced transgene, EGFP. (B) The recovered expression level was measured by flow cytometry. (C) 5-AzadC demethylated the methylated CMV promoter region. Values noted by a–h indicate they are significantly different. Data are the means± S.E.M. (<i>n</i> = 3).