RIND-EDSBs and chromatin regulators.

<p>(A) The levels of RIND-EDSBs were measured in G0 cells of yeast strains lacking histone deacetylase genes, <i>SIR2, RPD3</i>, and <i>HDA1</i>. The level was decreased in the <i>sir2</i>Δ strain, while it was increased in <i>rpd3</i>Δ strain. (B) No significant change in the level of RIND-EDSBs was observed in yeast strains lacking the silent information regulator genes, <i>SIR1</i>, <i>SIR3</i>, or <i>SIR4</i>. (C) In contrast, deletions of <i>HTZ1</i> and <i>SWR1</i>, genes required for the prevention of heterochromatin spreading, led to significantly increased levels of RIND EDSBs. The values from 9 independent experiments are shown as box plots, with the boxes representing the interquartile ranges (25^th to 75^th percentile) and the median lines representing the 50^th percentile. The whiskers represent the minimum and the maximum values. <i>**P</i><0.001 (Mann-Whitney test).