Histone H3-methylation patterns during somatic embryogenesis development in <i>Coffea canephora</i> using Chromatin Immunoprecipitation (ChIP).

<p>Samples were collected at the beginning of embryogenic induction (0d) and 14 days after embryogenic induction (14d) and during the proembryogenic mass (Pm), torpedo stage (T) and cotyledonary stage (C). The samples were examined for the Histone H3-tail methylation patterns, and the <i>LEC1</i>, <i>BBM1</i> and <i>WOX4</i> genes. Input (Input DNA): 10-fold diluted samples were used as templates for the input lanes. As negative control (-Ab), no antibody samples were treated in the same way as immunoprecipitated chromatin with H3K4me3, H3K9me2, H3K27me3 and H3K36me2. Amplified <i>UBQ11</i> with specific primers was used as the control for the quality of samples and the same amounts was used to amplify <i>LEC1, BBM1</i> and <i>WOX4</i>.