Ethanol down-regulates MyoD and Myogenin during C2C12 differentiation.

<p>C2C12 cells were induced to differentiate in DM +/−100 mM ethanol for 3 days. (A) MyoD and (B) myogenin mRNA levels were measured by qRT-PCR every 24 hours; transcript levels were quantified using the standard curve method and normalized to B-Actin. Black bars represent control medium treated cultures and grey bars represent cultures treated with medium containing alcohol. Mean ± SEM expression relative to non-alcohol control for each day are shown. *p≤0.05 versus control (n = 9 independent replicates).