Larvae of <i>A. cantonensis</i> developed in complete medium that contained 20% FCS.

<p>(a) Early third-stage larva. (b) Middle third-stage larva, showing evacuation of gut (arrow). (c) Late third-stage larva, showing expansion and evacuation of gut (arrow). (d) Fourth-stage larva enclosed in sheath (arrow). (e) Anterior end of fourth-stage larva, showing rod-shaped structures retained in sheath (arrow). (f) Exsheathing fourth-stage larva. (g) Exsheathed fourth-stage larva. (h) Comparison of sizes of fourth-stage larva from <i>in vitro</i> culture and third-stage larva of <i>A. cantonensis</i>. Fourth-stage larva on day 36 of culture (lower right-hand side) is significantly larger than third-stage larva (upper left-hand side). Bar = 0.25 mm.