ZLN024 activates human AMPK α1 subunits and inhibits AMPK dephosphorylation by PP2Cα.

<p>(A) The activation curve for the effect of ZLN024 on the activity of the AMPKα1 truncations (1–394) (n = 2), (B) α1 (1–335) (n = 2) and (C) α1 (1–312) (n = 2). (D) The effect of ZLN024 on PP2Cα (n = 3). (E) ZLN024 inhibits AMPK α1β1γ1 (n = 5) and AMPK α2β1γ1 (n = 3) (F) dephosphorylation by PP2Cα. The ratio of the phosphorylation level to the protein level of AMPK was determined. NC = negative control, PP2Ca was not added. (G) The additive activation of AMPK α2β1γ1 by different concentrations of ZLN024 and 12.5 µmol/l AMP (n = 2). (H) The additive activation of different concentrations of AMP and 10 µmol/l ZLN024 (n = 2). #, <i>P</i><0.1, *, <i>P</i><0.05, **, <i>P</i><0.01 compared with the untreated control.