Time-course of SMF cytotoxicity.

<p>Viability of SMF-exposed lymphocytes: (a) Trypan Blue dye exclusion assay; (b) MTT assay; data are reported as percentage of viability of unexposed lymphocytes at T0, taken as 100%. (c) Lysosomal activity of SMF-exposed lymphocytes, NR assay. (d) ROS production by SMF-exposed lymphocytes, NBT assay. Error bar represents the SE of six independent experiments, each done in duplicate. Star indicates significant value with respect to unexposed and NMD-exposed cells (p<0.0167). Control value is the average of the values of unexposed cells cultured by using either flasks or Petri dishes. Ctrl = unexposed lymphocytes; O.D. = optical density; h = hours.