Quantitative difference in Raman intensity between differentiated muscle/endoderm and daughter cells with other fates.

<p>(A–E) Raman intensities of A6.1, A6.2, B6.1, and B6.2 blastomeres, were acquired from 32-cell stage embryo (four individuals), and intensities of A7.5, A7.6, B7.3, B7.4, B7.7 and B7.8 blastomeres were acquired from 64-cell stage embryos (five individuals). Cell fate and embryonic location of each blastomere are shown in Fig. 4. Student's t-test results show significant differences for the intensities of Raman bands at 1002 (A and B) and 1526 (C–E) cm⁻¹ in Raman spectra of differentiated muscle/endoderm and other fate daughter cells (*p<0.005, **p<0.001). Error bars represent the standard error of the mean (SEM).