Genomic context of lectin-like proteins and phylogenetic distribution of expanded gene families in microsporidia.

<p><b>A</b>. Putative lectin-like proteins are shown in their genomic context as blue arrows and unrelated flanking genes are shown as grey arrows. Above these (white rectangles) are shown hypothetical translated proteins (not to scale) to illustrate the positions of predicted motifs. The proteins SLOPH 691 and 1766 that were identified in our secretion proteomics were BLASTed against the <i>Spraguea lophii</i> genome at a cutoff of e<1×10⁻⁵ to identify other members of the lectin-like family. These proteins also show BLASTP similarity to lectin-like proteins in <i>E. cuniculi</i> and <i>N. ceranae</i>, as indicated. <b>B</b>. RAxML 8 protein phylogeny of microsporidia showing the phylogenetic context of <i>S. lophii</i>. Published expanded gene families were mapped onto this phylogeny, including InterB proteins <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003676#pgen.1003676-Dia1" target="_blank">[66]</a>, leucine rich-repeat proteins (LRR) <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003676#pgen.1003676-Heinz1" target="_blank">[8]</a>, and other expanded gene families (EGF) as published <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003676#pgen.1003676-Cuomo1" target="_blank">[22]</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003676#pgen.1003676-Cornman1" target="_blank">[23]</a>. Stars indicate the detection of a single homolog of the protein in the genome. Shading is used to denote different families of apparently unrelated LRR proteins. Solid circles on branches indicate a posterior probability of 1 in our Bayesian phylogenetic analysis.