Acute knockdown of Rpl22 enhances Rpl22l1 expression.

<p>3T9 cells were transduced with doxycycline-inducible shRNA lentiviral constructs directed at <i>Rpl22</i> (shRNA 1 and shRNA 2) or a non-specific (ns) shRNA construct and treated with doxycycline for 3 days to induce shRNA expression. NS, shRNA 1, or shRNA 2 expressing cells were analyzed for relative <i>Rpl22</i> and <i>Rpl22l1</i> mRNA levels by qRT-PCR (<b>A</b>) or protein expression by Western blot analysis (<b>B</b>). Results are the average ± SEM of 4 independent experiments (<b>A</b>) or representative of 3 independent experiments (<b>B</b>). Statistical significance is indicated (*; p<0.001 compared to untreated control).