Increased alginate production in three mutants of CF149 with an <i>algU</i>-suppressor mutation.

<p>(A) Schematic diagram showing the transposon insertions of CF149 (+<i>algU</i>), CF149 (+<i>sspA</i>), and CF149 (<i>−rpoD</i>), respectively. (B) Alginate production of CF149 (+<i>algU</i>), CF149 (+<i>sspA</i>), and CF149 (<i>−rpoD</i>) in comparison to other strains of <i>P. aeruginosa</i>. Three mucoid mutants were identified as a result of a transposon library screen. Alginate production was measured on PIA plates after incubation at 37°C for 24 hrs. Alginate production (µg/ml/OD₆₀₀) was measured as described in Materials and Methods. M and NM, represent mucoidy and nonmucoidy, respectively.