Disruption of H2A.Z acidic patch increases chromatin dynamics in a transcription-dependent manner.

<p>(A) qRT-PCR results showing a decrease in the transcript levels of active genes- <i>Oct4</i>, <i>Tubb5</i>, and <i>Actin</i>, upon treatment of 1 µM flavopiridol and restoration of transcript levels upon removal of the agent at the various time points in H2A.Z^WT ESCs. (B–D) Graphs representing the estimated mobile fractions in H2A (B), H2A.Z^WT (C), and H2A.Z^AP3 (D) expressing ESCs upon treatment and removal of flavopiridol. ESCs treated with DMSO were used as a control. P values were generated by standard unpaired Student's t-test. *p<0.03, **p<0.015 and NS indicates p values >0.6. Quantitative PCR on ChIP DNA generated by using GFP antibodies in H2A.Z^AP3 (E) and H2A.Z^WT (F) ESCs treated with DMSO, 1 µM flavopiridol (Flavo) and 2 hrs after flavopiridol removal (Wash). Student's t-test was performed to test for statistical significance. * p<0.03, ** p<0.01, and NS represents not significant (>0.05). Error bars represent standard deviations from a triplicate set of experiments.