Cyp26b1-deficient T cells fail to promote intestinal inflammation following adoptive transfer into <i>Rag1^−/−</i> mice.

<p>CD4⁺CD45RB^highCD25⁻ naïve effector T cells from <i>Cyp26b1</i>^fl/fl or <i>Cyp26b1</i>^−/− mice were transferred i.p. into <i>Rag1</i>^−/− mice. (<b>A</b>) Weight loss was monitored over the entire disease course. (<b>B</b>) Histological sections of proximal colons stained with hematoxylin and eosin were scored for pathology and colons were scored for gross pathology. Cells from spleens and mesLNs were polyclonally stimulated overnight and stained for (<b>C</b>) IL-17a and (<b>D</b>) Foxp3 and CD25, then measured by flow cytometry. Gene expression of (<b>E</b>) <i>Cd4</i>, (<b>F</b>) <i>Il17a</i>, (<b>G</b>) <i>Ifng</i> and <i>Tnfa</i>, and (<b>H</b>) <i>Foxp3</i> (normalized relative to <i>Actb</i>) in proximal colons was measured by qRT-PCR. Data in (<b>A–H</b>) are representative of one of 2 independent experiments (n = 7−9 per experiment).