The effect of Mixture 1 on steroid hormone levels in H295R cells.

<p>Cells were incubated with Mixture 1 at concentrations ranging from 0.04 to 30 µM for 48 h. Hormones levels were measured by LC-MS/MS, except for estradiol that was measured by DELFIA. The figure shows the results of progesterone, 17-OH-progesterone, cortisol, dehydroepiandrosterone, androstenedione, estrone, testosterone and estradiol arranged according to appropriate steps in steroidogenesis. Data are mean ± SD expressed as per-cent of the control level. A <i>p</i>-value of less than 0.05 was considered significant, and in case of significance a sigmoidal curve fit (black line) was applied with a 95% confidence band (black dotted lines). Enzymes involved in steroidogenesis are illustrated by colour shaded boxes at appropriate steps. Abbreviations are as follows: CYP: Cytochrome P450, HSD: Hydroxysteroid dehydrogenase.