Global transcript profiling reveals gene expression changes in MYC-family pathways.

<p>(<b>a</b>) qRT-PCR confirmation of changes in <i>MYCN</i> and <i>MYC</i> expression following treatment with the indicated concentration of I-BET726. Data represent mean value ± standard deviation for three independent biological replicates. Asterisks indicate statistical significance as measured by t-test (p <0.05). (<b>b</b>) GSEA enrichment plots showing the down-regulation of gene sets associated with Myc/Max and N-Myc binding motifs in I-BET726-treated CHP-212 cells. Normalized enrichment scores (NES) and FDR q values are indicated. (<b>c</b>) A <i>MYCN</i> transcriptional regulation network was constructed (see Materials and Methods) to depict N-Myc pathway genes that were modulated by I-BET726 treatment. Red and blue circles represent increased and decreased expression changes, respectively. Green, red and grey edges are shown for activation, inhibition and unspecified interaction types, respectively. (<b>d</b>) qRT-PCR confirmation of a subset of genes selected from the N-Myc network analysis described in (c). Data presented as described in (a).