Immunohistochemical triple stainings to quantify excitatory synapses on GABAergic neurons in lamina II of the spinal dorsal horn.

<p>Confocal images depicting co-staining of GAD67 (green) with the postsynaptic marker PSD-95 (blue) and the presynaptic marker synaptophysin (red). Bottom right, white arrowheads in enlarged boxed areas indicate colocalization of the three antigens. Scale bars: 10 µm (2 µm for enlarged boxes).