HSC myeloablative stress response is not affected by deletion of all piwi genes.

<p>(A) White blood cells (WBC) are responsive to 5FU in both piwi triple knockout (blue circle) and control (black triangle) donor-derived cells. (B) Piwi triple knockout (blue circle) and control (black triangle) donor-derived cells (CD45.2⁺) show similar response kinetics following 5FU, measured up to 31 days after 5FU treatment. (C-E) Ratio of donor/recipient (CD45.2⁺/CD45.2⁻ ) derived cells from <i>Piwi</i> triple knockout (blue circle) and control (black triangle) donors in mature cell populations of recipient peripheral blood. (C) T-cell (CD3⁺), (D) B-cell (B220⁺), and (E) myeloid cell (CD11b⁺) lineages. 5FU injection point is indicated as a black arrow on each graph. Data is the average of 10 triple <i>Piwi</i> mutant injected recipients and 9 control injected recipients. Each data point represents the mean ±SEM of 8–10 recipient mice, per donor genotype.