Different combinations of IFN-α, RBV, and IFN-λ inhibits HCV IRES Rluc mediated translation.

<p>Huh-7 cells were infected with T7-expressing adenovirus. After 2 hrs, HCV IRES-RLuc plasmid was transfected and then treated with indicated concentration of IFN-α, IFN-λ and RBV. The concentration dependent inhibition of <i>Renilla</i> luciferase activity by (<b>A</b>) IFN-α, RBV, and IFN-λ single treatment;(<b>B</b>) Combination of IFN-α + IFN-λ; (<b>C</b>) Combination of IFN-α + RBV and (<b>D</b>) Combination of IFN-λ + RBV.