<p>Gel filtration on Sephadex G-200 of native and Hb at 20% and 70% glyoxal [▪]. The molecular weight marker such as urease, myosin, glucose oxidase [GOD], Concanavalin A [con A], bovine serum albumin [BSA], Hb, α1 antitrypsin, ovalbumin, chymotrypsin (Chy) were labelled by [♦<b>]</b>. The protein concentration was 30 µM. The tetrameric and dimeric forms of Hb were eluted in phosphate buffer of pH 7.</p
<p>(A) Electrophoresis of the purified protein on a 15% SDS-polyacrylamide gel. Lane 1: molecular w...
<p>Ve/Vo: Elution volume/Void volume ratio in gel filtration chromatography (superdex 200 16/60).</p...
<p>Marker: Unstained Protein Molecular Weight Marker; Lane 1: supernatant of culture fluid; Lane 2: ...
Dimeric bovine hemoglobin (Hb) tetramers were prepared by a one-step solid phase adsorption method. ...
(A) HepG3 protein separation was performed by preparative gel filtration on a Superdex 200 prep grad...
<p>(<b>a</b>) Hb aggregates at 70% glyoxal (<b>b</b>) AGE–Hb incubated at 30% glyoxal on day 20. The...
A solid phase adsorption method was proposed to prepare well-defined bovine serum albumin-bovine hem...
<p>Low molecular weight fraction of the hydrolysate (300 mg, less than 3 kDa) was separated by the B...
<p>Protein elution profiles are shown from the analytical Superose 6 10/300 GL column gel permeation...
<p>The protein was obtained in high yields (about 30 mg/L of culture) as insoluble inclusion bodies ...
(A) Chromatography of the DEAE-cellulose TP fraction on Sephadex G-150 column (B) Estimation of the ...
<p>Determination of hydrodynamic radii (R<sub>h</sub>) of (<b>a</b>) native Hb in absence of glyoxal...
<p>Soret Absorption spectra of native Hb in absence of glyoxal (curve 1); at 20% (curve 2); 70% (cur...
AbstractGlyoxalase I and glyoxalase II (EC. 4.4.1.5 and EC 3.1.2.6) were separated by gel filtration...
<p>(B) SDS PAGE of protein eluted from 5 step purification of cytosolic (ARG I) of <i>H. fossilis</i...
<p>(A) Electrophoresis of the purified protein on a 15% SDS-polyacrylamide gel. Lane 1: molecular w...
<p>Ve/Vo: Elution volume/Void volume ratio in gel filtration chromatography (superdex 200 16/60).</p...
<p>Marker: Unstained Protein Molecular Weight Marker; Lane 1: supernatant of culture fluid; Lane 2: ...
Dimeric bovine hemoglobin (Hb) tetramers were prepared by a one-step solid phase adsorption method. ...
(A) HepG3 protein separation was performed by preparative gel filtration on a Superdex 200 prep grad...
<p>(<b>a</b>) Hb aggregates at 70% glyoxal (<b>b</b>) AGE–Hb incubated at 30% glyoxal on day 20. The...
A solid phase adsorption method was proposed to prepare well-defined bovine serum albumin-bovine hem...
<p>Low molecular weight fraction of the hydrolysate (300 mg, less than 3 kDa) was separated by the B...
<p>Protein elution profiles are shown from the analytical Superose 6 10/300 GL column gel permeation...
<p>The protein was obtained in high yields (about 30 mg/L of culture) as insoluble inclusion bodies ...
(A) Chromatography of the DEAE-cellulose TP fraction on Sephadex G-150 column (B) Estimation of the ...
<p>Determination of hydrodynamic radii (R<sub>h</sub>) of (<b>a</b>) native Hb in absence of glyoxal...
<p>Soret Absorption spectra of native Hb in absence of glyoxal (curve 1); at 20% (curve 2); 70% (cur...
AbstractGlyoxalase I and glyoxalase II (EC. 4.4.1.5 and EC 3.1.2.6) were separated by gel filtration...
<p>(B) SDS PAGE of protein eluted from 5 step purification of cytosolic (ARG I) of <i>H. fossilis</i...
<p>(A) Electrophoresis of the purified protein on a 15% SDS-polyacrylamide gel. Lane 1: molecular w...
<p>Ve/Vo: Elution volume/Void volume ratio in gel filtration chromatography (superdex 200 16/60).</p...
<p>Marker: Unstained Protein Molecular Weight Marker; Lane 1: supernatant of culture fluid; Lane 2: ...