Soret spectroscopy.

<p>Soret Absorption spectra of native Hb in absence of glyoxal (curve 1); at 20% (curve 2); 70% (curve 3), and 30% glyoxal on day 20 (curve 4). The protein concentration was 3 µM and range was 350–700 nm. <b>(b)</b> Soret Absorbance of Hb at 410 nm in 20 mM sodium phosphate buffer, pH 7 as a function of increasing concentration of glyoxal. The protein concentration was 3 µM. Error bars represent the mean ± SD (n = 3). *Significance p<0.05 with respect to control. <b>(c)</b> Soret Absorbance of Hb at 410 nm in 20 mM sodium phosphate buffer, pH 7 as a function of increasing days in the presence of 30% [♦<b>]</b> and 40% [▪] glyoxal. The protein concentration was 8 µM. Error bars represent the mean ± SD (n = 3). *Significance p<0.05 with respect to control.