The Anxa1 as a transcriptional target of FoxM1.

<p><b>A,</b> transactivation of the Anxa1 promoter in SW1088-FoxM1 cells (left) and repression of the Anxa1 promoter in U-87MG-RNAi cells (right). Activation was calculated relative to SW1088 cells and inhibition was calculated as a percentage relative to U-87MG cells. Three independent experiments were conducted. <b>B,</b> Sequence and position of putative FoxM1 binding site on the Anxa1 promoter. <b>C,</b> ChIP assays were done with SW1088, SW1088-FoxM1, U-87MG and U-87MG-RNAi cells. Chromatin fragments of the cells were immunoprecipitated with anti-FoxM1 antibody (top) or negative control IgG (middle) and subjected to PCR. We subjected 1% of the total cell lysates to PCR before immunoprecipitation as inputs (bottom). <b>D,</b> schematic structure of the Anxa1 promoter. The sequence of the FoxM1 binding site is shown in both wild-type (WT) and mutant (Mut) forms. <b>E,</b> Luciferase activity with or without mutation in Anxa1 promoter. SW1088-FoxM1, U-87MG-RNAi, controls and parental cells were transfected with the wild-type Anxa1 promoter or its mutant. Three independent experiments were conducted. *<i>P</i><0.01.