1F7 binding is independent of the gp120 variable loops V1/V2 and V3.
- Johannes S. Gach (244457)
- Heribert Quendler (108436)
- Tommy Tong (426980)
- Kristin M. Narayan (105322)
- Sean X. Du (105326)
- Robert G. Whalen (105336)
- James M. Binley (426982)
- Donald N. Forthal (372785)
- Pascal Poignard (244468)
- Michael B. Zwick (105335)
Publication date
August 2013
DOIAbstract
<p>Binding of 1F7 to recombinant gp120<sub>JR-FL</sub> and its truncation mutants in a direct ELISA shows that 1F7 binding is independent of V1, V2, and V3. (A) 1F7 binding to wild type gp120<sub>JR-FL</sub>, (B) the variable loop truncated variants gp120<sub>JR-FL</sub> ΔV1/V2, and (C) gp120<sub>JR-FL</sub> ΔV3. Antibodies b12, VRC01, F105, 15e, 2G12, and HIVIG served as positive controls for each gp120, and F425-b4e8 to V3 is a negative control for the ΔV3 construct. Each curve is representative of at least three independent experiments performed in duplicate.</p
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