Isolation of novel MSCs from mouse umbilical cord.

<p>(A) Mouse UC-MSCs isolated from GFP transgenic mice at passage numbers 0 (Div 4) and 10 (P10). mUC-MSCs displayed the triangular, spindle-shaped, and fibroblast-like morphology at P10. (B) Growth Kinetics of mUC-MSCs. Cumulative cell number was counted at each passage. (C) The population doubling time pattern of mUC-MSCs from passage 10 to passage 50. (D) RT-PCR analysis of the pluripotency-associated genes in mUC-MSCs. Lane 1, mES cells; lane 2, germ cells from testis; lane 3, mUC-MSCs from heterogeneous populations (Mix); land 4-5, mUC-MSCs derived from different single colony. Scale bar = 50 µm.