Live imaging and fluorescence quantification of <i>Pb</i>red during blood infection.

<p>(A) Live cell imaging of <i>Pb</i>red c₅₀₇ -infected erythrocytes during blood stage development. Representative differential interference contrast DIC (left) and live fluorescent images for mCherry (center) and the green fluorescent proteins GFP (right) of <i>Pb</i>red-infected erythrocytes at different stages of development are shown. Nuclear stains (Hoechst) are visualized as merge in the DIC images. Life cycle stages are indicated on the left. Scale bars, 5 µm. (B) Quantitative analysis of mCherry (red) and GFP (green) fluorescence in different blood stages. Fluorescence intensities are presented in mean of grey values (± S.E.M.) for rings, trophozoites and gametocytes. *, <i>P</i><0.05 (students t-test).