Copper lowering in vivo promotes CD4⁺ T cell infiltration.

<p>Mice treated with PBS, TM, penicillamine or trientine were sacrificed when tumors reached 100 mm² and immune cell infiltration analyzed by flow cytometry. The SSC/FSC-A plot revealed the lymphocyte population (A). CD3⁺ regions were determined using the CD3-APC-Cy7 single stain (B). Triple staining identified CD3⁺CD4⁺ or CD3⁺CD8⁺ cells; representative contour plot (C). Pooled data from 2 mice/group shown as mean ± SEM (D). * p < 0.05.