Design of centered–site miRNA mimics (CS–miR-Mimics), seed–site miRNA mimics (SS–miR-Mimics), and siRNAs targeting BCL2 (a) and AKT1 (b) genes, respectively.

<p>The centered sites (nucleotides 5–16 from 5′–end) are indicated by underlined blue letters in boldface, and the seed sites (nucleotides 2–8 at 5′–end) by boldface, red letters and the 3′–complementary sites (nucleotides 13–16 from 5′–end) by boldface, purple letters. GS: guide strand; PS: passenger strand.