Increased PVN <i>Trh</i> mRNA content in mid-pregnant WT but not in <i>Il6</i>-KO mice.

<p>12 weeks old WT and <i>Il6</i>-KO mice were time-pregnant and PVN gene expression was assessed by radioactive in situ hybridization in coronal brain sections from early (n = 8–10), mid (n = 7–12) and late (n = 12–15) pregnant mice. Age and genotype matched virgin females were used as controls (n = 7–15). <b>A–C</b>. <i>Crh</i> (A) and <i>Trh</i> (B) mRNA levels were normalized in percentage to WT virgin control values and expressed as mean ± SEM (right panel). Representative i<i>n situ</i> hybridization autoradiographic images are shown in the left panel. One-way ANOVA, *P<0.05, **P<0.01 and ****P <0.0001.