PZA effect on monocytes stimulated with selected TLR agonists

<p>. Human monocytes treated with PZA (10 or 50 µg/ml) were simultaneously stimulated with (A) TLR4 (LPS, 100 ng/ml) or (B) TLR2/6 (Pam2, 250 ng/ml) and TLR2/1 (Pam3, 250 ng/ml) agonists. Pro-inflammatory and down-regulatory mediators were measured in the culture supernatants at 24 hours post-stimulation. Data are from 4 – 7 independent experiments (independent donors; N = 4 – 7) performed in duplicate and presented as percentage induction relative to PZA-untreated TLR-stimulated cells ± SD. * statistically significant; <i>P</i> ≤ 0.05 compared with the PZA-untreated TLR-stimulated cells.