Autophagy could be significantly induced by chLym-1 in Raji cells.

<p>A: Autophagy-related protein LC3-II is significantly up-regulated in Raji cells treated with chLym-1. Raji cells were treated with 10 µg/ml of chLym-1 for 24 h as described, while vehicles were treated with complete medium. Statistics was applied to detect relative intensities of LC3-II (LC3-II/actin). B: ChLym-1 induces autophagosomes accumulation (arrows) in Raji cells. Raji cells were treated with/without 10 µg/ml of chLym-1 for 24 h, and then prepared for transmission electron microscope. N = Nuclear. C: Appearance of autophagosome membrane-associated LC3-II in Raji cells treated with chLym-1. Raji cells were treated with/without chLym-1 for 24 h, and vehicles were treated with RMPI1640 supplemented with 10% fetal bovine serum (FBS). Raji cells treated with 50 nM of Rapamycin for 6 h were used as positive control. Spots were quantified by IQuantTL (GE Health Care). D: chLym-1 induces autophagy via autophagosomes accumulation but not via inhibition of autophagosomes degradation. Raji cell were treated with chLym-1 and/or 10 mM of Chloroquine (CQ) for 24 h.