Viral polyadenylated RNA is a major target of slicing by Ago2.

<p>(<b>A,B</b>) Levels of genome RNA, antigenome RNA, and polyadenylated virus RNA from <i>Dcr-2</i> mutants (hollow bars) and wildtype controls (solid bars) at different times post infection with VSV (<b>A</b>) and SINV (<b>B</b>). Asterisks indicate <i>p</i><0.05 comparing RNA levels between mutant and wildtype samples. (<b>C</b>) Fold increase in polyadenylated viral RNA, genome RNA, and antigenome RNA in <i>Dcr-2</i> mutants relative to wildtype at different times post infection with VSV or SINV. (<b>D</b>) VSV RNA levels in null <i>Ago2⁴¹⁴</i>, <i>Ago2^V966M/Ago2⁴¹⁴</i>, and wildtype heterozygous animals at different days post infection. Asterisks indicate <i>p</i><0.05 comparing RNA levels between mutant and matched wildtype samples; (@) indicates <i>p</i><0.05 comparing RNA levels between <i>Ago2⁴¹⁴</i> and <i>Ago2^V966M/Ago2⁴¹⁴</i> mutants.