Identification and functional validation of genomic regions that regulate PPARγ expression <i>in vivo</i>.

<p>(<b>A</b>) Comparative analysis of 100 Kb of sequence upstream of the PPARγ2 transcriptional start site reveals 5 elements that are highly conserved across multiple mammalian species (indicated as CS1 to 5 in the UCSC genome browser schematic). (<b>B</b>) X-gal staining of subcutaneous (SubQ), visceral (Visc), and retroperitoneal (Retro) WAT, brown adipose tissue (BAT), and other organs from wild type, PPARγ (+/−), and PPARγ CS1-5_<i>LacZ</i> line 1 transgenic mice (6 weeks). Note that CS1 to 5 drive reporter expression in a similar tissue-specific pattern to that of <i>LacZ</i> expressed from the endogenous PPARγ locus. (<b>C</b>) X-gal staining of wild type, PPARγ (+/−), and PPARγ CS1-5_<i>LacZ</i> line 1 and 7 embryos at E14.5.