Flash responses from wild type, Gα_GTP^G38D+/−, and Gα_GTP^G38D−/− rods.

<p>Experimental (A, B, C) <i>versus</i> simulated (D, E, F) responses to flashes of increasing intensities from wild type Gα_GTP^WT+/+ (A, D), heterozygous Gα_GTP^G38D+/− (B, E) and homozygous Gα_GTP^G38D−/− (C, F) rods are shown. Experimental data, i.e. published in Moussaif et al. <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003207#pcbi.1003207-Moussaif1" target="_blank">[8]</a> and provided by Marie E. Burns, refer to mice rods exposed to flashes ranging from 5 to 97000 photons µm⁻² (A and B) or from 650 to 94000 photons µm⁻² (C). Simulated responses derive from the model of an amphibian rod stimulated with the same light intensities as <i>in vitro</i> recordings. The pathological Gα_GTP^G38D+/− and Gα_GTP^G38D−/− models were generated by changes in the kinetic parameters <i>kP1</i>, <i>kP2</i> and <i>kRGS1</i> as described in the text. The dissimilar species justify the time scale difference between <i>in vitro</i> and <i>in silico</i> experiments. The responses were normalized with respect to the maximum photocurrent. <b>G.</b> Normalized simulated light response amplitude is plotted as a function of flash strength. For comparison to <i>in vitro</i> data, see <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003207#pcbi-1003207-g005" target="_blank">Figure 5B</a> in Moussaif et al. <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003207#pcbi.1003207-Moussaif1" target="_blank">[8]</a>. Flash intensities are the same as in D, E and F.