Influence of <i>Drosophila melanogaster</i> larval grazing on <i>Aspergillus nidulans</i> gene expression.

<p>Gene expression differences between wild type <i>A</i>. <i>nidulans</i> exposed to <i>D</i>. <i>melanogaster</i> larval grazing (filled bars) and unchallenged (open bars) colonies. Mean values are shown for initial SYBR green fluorescence amount (R₀) proportional to initial amount candidate gene mRNA in the qRT-PCR runs (N = 5 per treatment). Colours represent different molecular “modules” involved in determining the fungal phenotype: transcription regulation (dark red), secondary metabolite biosynthesis (grey), oxylipin formation (green), signal transduction (violet) (see Figure 2 for details). Data were transformed to eliminate gene-specific variation in expression differences and to achieve normality and variance homogeneity; see Methods for details and Figure S2 for untransformed gene expression data. Statistics refer to results of the between-subject effect analysis of the multivariate nested analysis of variance on ranks; <i>Drosophila</i> larval grazing had an overall effect on fungal gene expression, P = 0.025.