Bioinformatics based predictions of the <i>C.</i><i>ureolyticus</i> secretome.

<p>(A) 288 proteins were predicted to be secreted by <i>C. ureolyticus</i> DSMZ 20703 using the SignalP 4.1 server for classically secreted proteins and the SecretomeP 2.0 server for non-classically secreted proteins. Of the 288 proteins, 187 proteins were predicted to be secreted by the SecretomeP 2.0 sever alone, 57 proteins by the SignalP 4.1 alone and 44 proteins were identified by both servers. Additionally, 117 proteins of 28 were hypothetical, the remaining 171 proteins were associated with a diverse range of functions relating to membrane transport, protein metabolism, respiration, stress response and at least 25 proteins with putative virulence roles. (B) SignalP4.1 and SecretomeP2.0 servers predicted 269 proteins are secreted by <i>C. ureolyticus</i> ACS-301-V-Sch3b. 165 proteins were predicted to be secreted by the SecretomeP 2.0 server alone, a further 65 proteins were identified by the Signal 4.1 server and 39 proteins were identified by both servers. Of the 269 secreted proteins, a total of 94 were hypothetical proteins, the remaining 175 proteins were predicted to have a wide range of functions including DNA metabolism, cell wall synthesis, sulphur and nitrogen metabolism and at least 28 proteins with putative virulence roles.