Increased podocytes death and reduced podocytes adhesion cultured with conditioned medium of mesangial cells exposed to cIgA1 from IgAN patients (IgAN-HMCM) and rhTGF-β1 together with rhCXCL1.

<p>A: The podocytes released LDH were significantly increased when podocytes were cultured with conditioned medium of mesangial cells exposed to cIgA1 from IgAN patients (IgAN-HMCM) compared to from healthy controls (HC-HMCM). B: Similar increased LDH could also be observed when podocytes were treated with 2ng/ml CXCL1 and 2ng/ml TGF-β1 together (symbol * represented p<0.05 compared with control). The increased podocytes released LDH, induced by IgAN-HMCM or rhTGF-β1 (2ng/ml) together with rhCXCL1 (2ng/ml) could be partially rescued by simultaneous incubation with 0.5ug/ml blocking antibody against CXCR2 (symbol # represented p<0.05 compared with without CXCR2 blocking antibody). C: The number of adherent podocytes were significantly decreased when podocytes were cultured with IgAN-HMCM compared to HC-HMCM (5790±377 cells <i>vs</i>. 9272±736 cells, P=0.002). D: Similar decreased podocyte adhesion could also be observed when podocytes were treated with 2ng/ml CXCL1 and 2ng/ml TGF-β1 together (7327±326 cells <i>vs</i>. 10468±499 cells, P=0.001) (symbol * represented p<0.05 compared with control). The decreased podocyte adhesion, induced by IgAN-HMCM or rhTGF-β1 (2ng/ml) together with rhCXCL1 (2ng/ml) could be partially rescued by simultaneous incubation with 0.5ug/ml blocking antibody against CXCR2 (symbol # represented p<0.05 compared to without CXCR2 blocking antibody).