Integration of <i>glmS</i> ribozyme sequence into the essential <i>Pf</i>DHFR-TS gene.

<p>(A) Schematic showing the strategy used to incorporate the GFP and <i>glmS</i> ribozyme sequences at the 3′ end of the <i>Pf</i>DHFR-TS gene by single crossover homologous recombination. The expected sizes of PCR products shown in part B are marked. (B) PCR testing of plasmid integration from two cloned transgenic lines, clone #1 and #2. PCR primer combinations used are indicated above the lanes and the migrations of 1 kb+ DNA marker (Invitrogen) bands are indicated on the left.