Involvement of PLAU in cumulus expansion and oocyte maturation.

<p>A, COC morphologies after IVM culture under varying conditions are shown: a, control COCs after 6 h of culture; b, control COCs after 6 h of culture; c, COCs incubated with 20 U of PLAU for 6 h; d, COCs incubated with 20 U of PLAU for 16 h; e, COCs incubated with 20 U of PLAU for 20 h; f, COCs incubated with 300 µM amiloride for 20 h; g, COCs coincubated with SERPINE2 (0.12 mg/ml) and PLAU (20 U) for 20 h; h, COCs coincubated with amiloride (300 µM) and PLAU (20 U) for 20 h. Scale bars, 100 µm. B, Cumulus expansion was measured as the COC diameters after 16 h of IVM (n = 100 each group). D, MII oocyte maturation rate after 16 h of IVM. B and C, Data represent means ± SD for six independent experiments; ^†<i>P</i><0.05, *<i>P</i><0.0001 vs. controls.