In-situ-zymography combined with MMP antigen detection.

<p>Control (B, D, F) and IPF (A, C, E, G) lung samples obtained in the surgical theatre were analyzed for collagenase activity (in-situ-zymography, green) and for MMP-1 (upper panel), -8 (middle) and -13 (lower panel) antigen signal (immunohistochemistry, red) within the same cryostat section. Nuclei were stained with DAPI (blue). White arrows show the co-localization of collagenolytic activity with: MMP-1 antigen signal in the septal and scar regions (A); MMP-8 antigen signal in septae (C); and MMP-13 antigen signal along the airways, alveolar epithelial cells and cells lining fibroblastic foci (E). Section G represents a negative control and shows an IPF section analyzed for collagenase activity in presence of 30mM captopril. Representative sections are given. Original magnification = 10X, 20X and 40X; scale = 100µm.