Immunization in the presence or absence of CAF01.

<p>(A) End-point binding titer of rabbits immunized with ITM1_4, in the presence (filled triangles) or absence (open triangles) of CAF01 respectively. Results are given for weeks 8, 12 and 14. Each dot represents one rabbit. Horizontal lines indicate mean titers. Statistical analysis was done using Mann-Whitney test. (B) Neutralization using IgG (at a final concentration of 250 µg/ml) isolated from week 12 (filled squares) and 14 (open triangles) sera of rabbits immunized with ITM1_4 in the presence or absence of CAF01. Two clade B viruses, SF162 (left) and Bx08 (right) were used. Horizontal lines indicate the mean percent neutralization. The dotted (green) line represents 50% neutralization. Statistical analysis was done using Mann-Whitney test.