Effects of IKKα KD on TIMP-3 levels in human OA chondrocyte micromass cultures.

<p>(<b>A</b>) Quantitative image analysis for TIMP-3 immunostaining of 1-week IKKα KD human pellet cultures vs. matched WT (GL2 control) for 3 OA patients. Results are shown as mean ± S.E.M. *p<0.05 by Student’s t-test. Representative images of TIMP-3 immunostained micromasses are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0073024#pone-0073024-g005" target="_blank">Figure 5</a>. (<b>B</b>) TIMP-3 immunoblot of whole cell lysates of IKKα KD and matched WT (GL2 control) of 1-week human chondrocyte pellet cultures of one representative OA patient, with β-tubulin as protein loading control. (<b>C</b>) qRT-PCR analysis of TIMP3 mRNA in 1-week pellet cultures of IKKαKD human chondrocytes vs. matched WT (GL2) controls (N = 6). Results are shown as mean ± S.E.M. ns = not significant.