Depletion of Tregs by anti-CD25 mAbs in CCL21-treated mice.

<p>Respectively on day 1, 3, 5, 7, 9 after treatment initiation in the independent treatment groups, mice were sacrificed and tumors were harvested for quantification of CD25⁺ Foxp3⁺ Tregs. (<b>A</b>) Flow cytometry analysis for the population of CD25⁺ Foxp3⁺ Tregs within the tumors on day 1 to 9 after treatment initiation. Representative percentages of CD25⁺ Foxp3⁺ Tregs were shown in density plots. (<b>B</b>) A curve diagram was summarized for the results from all test and control mice (n = 3 for each group). *<i>P</i> < 0.05, **<i>P</i> < 0.01, compared with the control group. Similar results were obtained in three independent experiments.