Methylene blue has no inhibitory effect on amylin monomers and dextran in RIN-m5F cells.

<p>Cells were first treated with macropinocytotic inhibitors (EIPA, CytD or Wort) for 1 hour followed by addition of human amylin (10 µM) either in the absence or in the presence of MB (100 µM) for 24 hours at 37°C. Dextran was added for an additional 30 minutes. Confocal microscopy revealed no significant change in the cellular distributions of amylin monomers (green) when treated with macropinocytotic inhibitors with or without MB (left and right panel). Also, in the presence of MB, dextran (red) turnover was unaffected (right panel). Bar 10µm.