Role of Na⁺ and K⁺ currents in basal and hypotonic-induced transepithelial current.

<p>Basal transepithelial current (<i>I</i>_<i>sc</i> Basal), hypotonic-induced current (<i>I</i>_<i>sc</i> 20% Hypo) and the current rise elicited by hypotonic shock (Δ <i>I</i>_<i>sc</i> Shock) are depicted in the basal condition (Ctrl), potassium-free condition (K⁺(-)) or after pretreatment with 1µM amiloride (Amil 1), 100 µM amiloride (Amil 100) or 100 µM basolateral clofilium (Clofi). In experiments where the cells were pre-treated with an inhibitor that impacted on <i>I</i>_<i>sc</i>, hypotonic shock was induced when the current was stabilized. For amiloride the current was stable after 5 min. In potassium-free condition (K⁺(-)) and clofilium pre-treatment, a longer incubation was needed (~30 min). N≥4, *p<0.05 by Mann-Whitney Test compared to untreated controls.