Effect of FGF-23 on <i>CYP27B1</i> mRNA expression in cultured mouse aortic vascular smooth muscle cells (VSMC).

<p><b>A.</b> Activation of ERK1/2 signaling pathway was demonstrated in VSMC treated with FGF-23 (100 ng/ml) for 5–60 min. Phosphorylated ERK1/2 protein expression was detected by western blot analysis. Total Erk2 protein expression was used as loading control. <b>B. </b><i>CYP27B1</i> mRNA expression in VSMC treated with FGF-23 (100 ng/ml) for 21 hrs. mRNA expression was quantitated by real-time PCR, normalized to that of gus mRNA, and expressed as a percent relative to vehicle-treated group. Bars depict mean±SEM (n = 3 separate experiments in triplicate) *<i>P<0.05</i>, compared to vehicle-treated group.