Pulmonary expression of <i>CYP27B1.</i>

<p><i>Fgf-23^+/+/</i>1α-Luc<i>^+/−</i> (wt), <i>fgf-23^+/−/</i>1α-Luc<i>^+/−</i> (het) and <i>fgf-23^−/−/</i>1α-Luc<i>^+/−</i> (ko) mice were bred as described in <i>Methods.</i> Mice were sacrificed at 4 weeks of age and the lung removed and divided into two parts for determination of <b>A.. </b><i>CYP27B1</i> promoter activity, expressed as luciferase activity per mg of tissue. Graph depicts fold change with respect to luciferase activity in <i>fgf-23^+/+/</i>1α-Luc<i>^+/−</i> mice. <b>B.. </b><i>CYP27B1</i> mRNA expression, quantitated by real-time PCR, normalized to that of gus mRNA, and expressed as a percent relative to <i>fgf-23^+/+/</i>1α-Luc<i>^+/−</i> mice. Bars depict mean±SEM (n = 5 mice/group), *<i>P<0.05</i>, compared to <i>fgf-23^+/+/</i>1α-Luc<i>^+/−</i> mice.