Quantifying Cdc–TAP protein levels expressed by a single chromosomal copy with an increase in gene copy number.

<p><b>A</b>. <i>S. pombe</i> strains for determining the increases in protein levels expressed by a single chromosomal copy with an increase in gene copy number. Each <i>cdc–</i>TAP strain was transformed with either an empty vector or the corresponding target plasmid and then cultured in medium with or without leucine (as indicated). <b>B</b>–<b>E</b>. Quantitative results for Cdc16–TAP, Sid2–TAP, Cdc10–TAP, and Cig1–TAP. TAP-tagged protein levels were determined as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0073319#s4" target="_blank">Methods</a>. Copy number* is the copy number of a Target plasmid plus 1 (chromosomal copy).