eEF2 kinase overexpression selectively inhibits IRES dependent translation independently of the uORF.

<p>Cultured <i>Aplysia</i> sensory neurons were injected with either empty expression vector (Con) or <i>Aplysia</i> eEF2K (eEF2K). Representative neurons (48 hours later) show cyan fluorescence (cap-dependent translation, Cap) and yellow fluorescence (IRES-dependent translation, IRES) in the same neurons. Histogram is the means (normalized to Con) and SEMs from the groups of the representative neurons. The ratio of IRES- to cap-dependent translation was calculated (IRES/Cap). Values, SEMS and ns are: Control (CAP 1.00 ± 0.09, IRES 1.00 ±0.07, IRES/CAP 1.00 ± 0.06, n =51 neurons from four experiments); eEF2K (CAP 1.28 ± 0.17, IRES 0.35 ± 0.02, IRES/CAP 0.35 ± 0.04, n=52 neurons from four experiments. P values from Student’s <i>t</i> test compared to control (with Welch’s and Bonferroni’s corrections) are shown over the bars.