Functional analysis of the <i>Hv-SGT1</i> gene by BSMV-induced gene silencing.

<p>(A) Following infection of <i>H</i><i>. villosa</i> with the virus, chlorotic mosaic symptoms were observed on the 4^th leaves 10 days after inoculation (dpi) with BSMV:γ or BSMV:<i>Hv-SGT1</i>. Photobleaching was observed on leaves infected with BSMV:<i>PDS</i> 15 dpi, whereas no detectable phenotype was observed in the mock-treated plants inoculated with 1×GKP buffer. Representative photographs were taken 15 days after virus inoculation. (B) The <i>Hv-SGT1</i> gene was efficiently silenced in the BSMV: Hv-SGT1 inoculated leaves compared to the control inoculated with BSMV:γ, as indicated using qRT-PCR analysis. (C) More infected <i>Bgt</i> could developed into secondary hyphae in the BSMV: Hv-SGT1 inoculated leaves compared to the BSMV:γ infected leaves 10 days after inoculation with <i>Bgt</i>. (D) The rate of formation of secondary hyphae (SH) was higher in the <i>Hv-SGT1</i> silenced leaves inoculated with BSMV:Hv-SGT1 compared with that in BSMV:γ-infected leaves at 5, 7, and 10 days after inoculation with <i>Bgt</i>. (E) Levels of <i>NADPHOX</i>, <i>APX</i>, <i>NPR1</i>, <i>PR5</i>, <i>OPDA</i>, <i>COI1</i>, <i>ERF1</i>, <i>PR10, PR2, PR3, GST</i>and <i>GPOX</i>in <i>Hv-SGT1</i> silenced leaves. * p < 0.05, ** p < 0.01 compared to the BSMV:γ-infected leaves.