Study design and immunization schedule.

<p>C57Bl/6 mice were immunized with three pentavalent DNA primes followed by two heterologous gp120 protein boost. The pentavalent vaccine mixture of both DNA and protein components consisted of HIV-1 Env from clades A (92UG037.8), B (92US715.6 and Bal), C (96ZM651), and E (93TH976.17). DNA and protein doses indicated are for a total of five immunogens at each immunization. Four adjuvants were tested individually as part of the protein boost. Time points of immunizations, and sample collections for different assays were indicated.